brain was removed from the skull after being perfused in
situ by 10% saline followed by a mixture of formol-saline
until the vessels were cleared of blood. The brain was allowed
to harden in the skull for several hours and then was carefully
removed from the skull so as to minimize damage to or compression
of the brain tissue. This was accomplished by first carefully
removing the surrounding skull by use of delicate rongeurs,
spatula, forceps and scissors.